Journal: Experimental and Therapeutic Medicine
Article Title: Hypermethylation of the κ1 opioid receptor promoter in Chinese heroin and methamphetamine addicts
doi: 10.3892/etm.2018.6514
Figure Lengend Snippet: Correlations between OPRK1 promoter methylation and phenotypic indices in male addicts. (A) Associations between drug time and DNA methylation in different populations. (B-D) Association between frequency of drug use and DNA methylation in (B) total, (C) heroin and (D) METH addicts. OPRK1, κ1 opioid receptor; METH, methamphetamine.
Article Snippet: The level of DNA methylation was measured using bisulfite pyrosequencing technology which combined sodium bisulfite DNA conversion chemistry (EZ DNA Methylation-Gold™ kit), polymerase chain reaction (PCR) amplification (Zymo Taq™ PCR PreMix; both from Zymo Research Corporation, Irvine, CA, USA) and sequencing by synthesis assay (Pyromark Gold Q24 Reagents; Qiagen, Hilden, Germany) as previously described ( ).
Techniques: Methylation, DNA Methylation Assay